Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization

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Authors

  • Daniele Belluoccio
  • Ivan Grskovic
  • Anja Niehoff
  • Ursula Schlötzer-Schrehardt
  • Sabrina Rosenbaum
  • Julia Etich
  • Christian Frie
  • Friedericke Pausch
  • Stephen E Moss
  • Ernst Pöschl
  • John F Bateman
  • Bent Brachvogel

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Details

Initiation of mineralization during endochondral ossification is a multistep process and has been assumed to correlate with specific interactions of annexins A5 and A6 and collagens. However, skeletal development appears to be normal in mice deficient for either A5 or A6, and the highly conserved structures led to the assumption that A5 and A6 may fulfill redundant functions. We have now generated mice deficient of both proteins. These mice were viable and fertile and showed no obvious abnormalities. Assessment of skeletal elements using histologic, ultrastructural, and peripheral quantitative computed tomographic methods revealed that mineralization and development of the skeleton were not significantly affected in mutant mice. Otherwise, global gene expression analysis showed subtle changes at the transcriptome level of genes involved in cell growth and intermediate metabolism. These results indicate that annexins A5 and A6 may not represent the essential annexins that promote mineralization in vivo.

Original languageEnglish
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume25
Issue number1
Pages (from-to)141-53
Number of pages13
DOIs
Publication statusPublished - 01.01.2010

ID: 582869

DOI

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