Detection of proteases and their proteolysis and autolysis products in urine by LC-MS

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch

Abstract

The use of peptide hormones for performance enhancement is documented by positive doping control samples with e.g. human chorionic gonadotropin or erythropoietin and by confession of athletes or confiscation of peptide hormone products. With the development of doping control methods, dopers start searching for manipulation and masking strategies to circumvent the detection of prohibited substances in urine samples. One way to eliminate peptide hormones from doping control samples is the use of proteases that degrade all proteins in urine and beside the proteolytic activity usually also autolyse. A method was established and validated to detect proteases and their degradation products in urine by SDS-PAGE as a screening procedure and LC-MS(/MS) for confirmation and for identifying a specific protease m the sample. Two doping control urine samples were shown to be positive for bacillolysin and four seized products for bacillolysin, subtilisin and aspergillopepsin. Verf.-Referat
Original languageEnglish
Title of host publicationRecent advances in doping analysis (17) : Proceedings of the Manfred Donike Workshop ; 27th Cologne Workshop on Dope Analysis, 1st to 6th March 2009
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages10
PublisherSportverlag Strauß
Publication date2009
Pages107-116
Publication statusPublished - 2009
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 01.03.200906.03.2009
Conference number: 27

Fingerprint

Dive into the research topics of 'Detection of proteases and their proteolysis and autolysis products in urine by LC-MS'. Together they form a unique fingerprint.

Citation