Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry

Publication: Contribution to journalJournal articlesResearch

Abstract

In the present study, a screening assay was developed comprising 11 prohibited peptides (<1.5 kDa) that are sufficiently purified from urine using weak cation exchange with subsequent determination of all substances by means of nanoUHPLC separation coupled to high resolution tandem mass spectrometry. These peptides included Gonadorelin (LH-RH), Desmopressin and 9 growth hormone releasing peptides (GHRP-1, -2, -4, -5, -6, Hexarelin, Alexamorelin, Ipamorelin and a GHRP-2 metabolite); however, the procedure is expandable to further target analytes or metabolites. The method was validated with a main focus on qualitative result interpretation considering the parameters specificity, linearity (0-500 pg/mL), recovery (45-95%), precision (<20% at 100 pg/mL), limits of detection (2-10 pg/mL), robustnesss and ion suppression. The proof-of-principle was shown by analysing excretion study urine samples for LHRH, Desmopressin and GHRP-2.

Original languageEnglish
JournalJournal of chromatography. A
Volume1259
Pages (from-to)251-257
Number of pages7
DOIs
Publication statusPublished - 12.10.2012

Research areas and keywords

  • Chromatography, Liquid
  • Doping in Sports
  • Female
  • Humans
  • Limit of Detection
  • Linear Models
  • Male
  • Nanotechnology
  • Oligopeptides
  • Reproducibility of Results
  • Substance Abuse Detection
  • Tandem Mass Spectrometry

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