Di(2-ethylhexyl) phthalate metabolites as markers for blood transfusion in doping control : intra-individual variability of urinary concentrations

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Abstract

To indicate homologous or autologous blood transfusion in sports drug testing, quantification of increased urinary concentrations of di(2-ethylhexyl) phthalate (DEHP) metabolites presents a promising approach; however, the possible intra-individual variation of the metabolite concentrations over time has not been well characterized. The aim of this study was to explore the intra-individual variability of urinary DEHP metabolites among seven volunteers without special occupational exposure to DEHP during one week (n = 253) in order to investigate the possibility of increased urinary concentrations of the metabolites caused by, for example, residential, dietary, or environmental exposure. Quantification of three DEHP metabolites – mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-oxohexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate – was accomplished after enzymatic hydrolysis of urinary glucuronide conjugates and direct injection using isotope-dilution liquid chromatography-tandem mass spectrometry. Although urinary concentrations of DEHP metabolites showed considerable intra-individual variation, no increased values were observed comparable to the concentrations measured in urine specimens collected after blood transfusion. Verf.-Referat
Translated title of the contributionDi(2-ethylhexyl)phthalat-Metaboliten als Marker für Bluttransfusionen in der Dopinganalytik : intraindividuelle Variabilität von Urinkonzentrationen
Original languageEnglish
JournalDrug testing and analysis
Volume3
Pages (from-to)892-895
Number of pages4
ISSN1942-7603
Publication statusPublished - 2011

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