Direct quantification of salbutamol in human urine by means of LC-MS/MS

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch

Abstract

According to WADA rules salbutamol concentrations in urine greater than 1 µg/ml are defined as a doping violation. Concentrations greater than 500 ng/ml should be reported as an adverse finding relative to use of a ß2 agonist (WADA Technical Document — MRPL revised version 2007). The threshold concentration is based on the sum of the glucuronide conjugate and free drug concentrations. Because of the time consuming effort of sample preparation, a relatively high concentration of salbutamol in the urine samples and adequate LC behaviour of salbutamol an alternative approach for quantitative determination of salbutamol in doping control samples was developed utilizing LC-MS/MS. The urine samples are fortified with deuterated salbutamol and without any further sample preparation, aliquots of 10 µl are injected into the LC-MS/MS instrument. Lower limit of detection was 20 ng/ml, interday precisions 14.1 % for low (200 ng/mL), 7.1 % for medium (500 ng/mL) and 4.8 % for high (1000 ng/mL) concentration. The intermediate precision was 7.7 % for the reporting threshold 500 ng/mL and 11.8 % for the 1000 ng/mL threshold, respectively. Verf.-Referat
Original languageEnglish
Title of host publicationRecent advances in doping analysis (15) : Proceedings of the Manfred Donike Workshop ; 25th Cologne Workshop on Dope Analysis 25th February to 2nd March 2007
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages10
PublisherSportverlag Strauß
Publication date2007
Pages87-96
Publication statusPublished - 2007
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 25.02.200702.03.2007
Conference number: 25

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