Does oral fluid contribute to exhaled breath samples collected by means of an electret membrane?

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@article{c164fa5a7f5642c0b865cc951ce268d8,
title = "Does oral fluid contribute to exhaled breath samples collected by means of an electret membrane?",
abstract = "To date, blood (and serum) as well as urine samples are the most commonly collected specimens for routine doping controls, which allow for the analytical coverage of an extensive set of target analytes relevant to sports drug testing programs. In the course of studies to identify potential alternative matrices to complement current testing approaches, exhaled breath (EB) has been found to offer advantageous properties especially with regard to the sample collection procedure, which is less invasive, less intrusive, and less time-consuming when compared to conventional blood and urine testing. A yet unaddressed question has been the potential contribution of oral fluid (OF) to EB samples. The current investigation focused on characterizing an electret membrane-based EB collection device concerning a potential introduction of OF during the sampling procedure. For that purpose, EB and OF samples collected under varying conditions from a total of 14 healthy volunteers were tested for the presence of abundant salivary proteins using bottom-up proteomics approaches such as SDS-PAGE followed by tryptic digestion and chromatographic-mass spectrometric analysis. The trapping baffles integrated into the mouthpiece of the EB collection device were found to effectively retain OF introduced into the unit during sample collection as no saliva breakthrough was detectable using the established analytical approach targeting predominantly the highly abundant salivary α-amylase. Since α-amylase was found unaffected by storage, smoking, food intake, and exercise, it appears to be a useful marker to reveal possible OF contaminations of EB collection devices.",
author = "Ann-Marie Garzinsky and Katja Walpurgis and Oliver Krug and Mario Thevis",
note = "{\circledC} 2019 The Authors Drug Testing and Analysis Published by John Wiley & Sons Ltd.",
year = "2019",
doi = "10.1002/dta.2597",
language = "English",
journal = "Drug testing and analysis",
issn = "1942-7603",
publisher = "John Wiley & Sons, Ltd",

}

RIS

TY - JOUR

T1 - Does oral fluid contribute to exhaled breath samples collected by means of an electret membrane?

AU - Garzinsky, Ann-Marie

AU - Walpurgis, Katja

AU - Krug, Oliver

AU - Thevis, Mario

N1 - © 2019 The Authors Drug Testing and Analysis Published by John Wiley & Sons Ltd.

PY - 2019

Y1 - 2019

N2 - To date, blood (and serum) as well as urine samples are the most commonly collected specimens for routine doping controls, which allow for the analytical coverage of an extensive set of target analytes relevant to sports drug testing programs. In the course of studies to identify potential alternative matrices to complement current testing approaches, exhaled breath (EB) has been found to offer advantageous properties especially with regard to the sample collection procedure, which is less invasive, less intrusive, and less time-consuming when compared to conventional blood and urine testing. A yet unaddressed question has been the potential contribution of oral fluid (OF) to EB samples. The current investigation focused on characterizing an electret membrane-based EB collection device concerning a potential introduction of OF during the sampling procedure. For that purpose, EB and OF samples collected under varying conditions from a total of 14 healthy volunteers were tested for the presence of abundant salivary proteins using bottom-up proteomics approaches such as SDS-PAGE followed by tryptic digestion and chromatographic-mass spectrometric analysis. The trapping baffles integrated into the mouthpiece of the EB collection device were found to effectively retain OF introduced into the unit during sample collection as no saliva breakthrough was detectable using the established analytical approach targeting predominantly the highly abundant salivary α-amylase. Since α-amylase was found unaffected by storage, smoking, food intake, and exercise, it appears to be a useful marker to reveal possible OF contaminations of EB collection devices.

AB - To date, blood (and serum) as well as urine samples are the most commonly collected specimens for routine doping controls, which allow for the analytical coverage of an extensive set of target analytes relevant to sports drug testing programs. In the course of studies to identify potential alternative matrices to complement current testing approaches, exhaled breath (EB) has been found to offer advantageous properties especially with regard to the sample collection procedure, which is less invasive, less intrusive, and less time-consuming when compared to conventional blood and urine testing. A yet unaddressed question has been the potential contribution of oral fluid (OF) to EB samples. The current investigation focused on characterizing an electret membrane-based EB collection device concerning a potential introduction of OF during the sampling procedure. For that purpose, EB and OF samples collected under varying conditions from a total of 14 healthy volunteers were tested for the presence of abundant salivary proteins using bottom-up proteomics approaches such as SDS-PAGE followed by tryptic digestion and chromatographic-mass spectrometric analysis. The trapping baffles integrated into the mouthpiece of the EB collection device were found to effectively retain OF introduced into the unit during sample collection as no saliva breakthrough was detectable using the established analytical approach targeting predominantly the highly abundant salivary α-amylase. Since α-amylase was found unaffected by storage, smoking, food intake, and exercise, it appears to be a useful marker to reveal possible OF contaminations of EB collection devices.

U2 - 10.1002/dta.2597

DO - 10.1002/dta.2597

M3 - Journal articles

C2 - 30927335

JO - Drug testing and analysis

JF - Drug testing and analysis

SN - 1942-7603

ER -

ID: 4432683