Abstract
PURPOSE: Gamma irradiation of red blood cell (RBC) concentrates is routinely used to prevent transfusion-associated graft-versus-host disease. So far, the effects of ionizing radiation on RBC structure and function and especially the proteome are not fully understood.
EXPERIMENTAL DESIGN: RBC concentrates were irradiated with 30 Gy and stored for 1 or 15 days at 4 ± 2°C. Following cell lysis and hemoglobin depletion, 2D-DIGE was used to examine the changes of the cytosolic RBC proteome. Significantly altered spots were analyzed using bottom-up proteomic approaches and selected marker proteins validated by western blotting.
RESULTS: Gamma irradiation was found to enhance conventional RBC storage lesions. Following 15 days of postirradiation storage, the abundances of a total of 27 spots were significantly altered and 3 out of 13 identified proteins were selected and validated as potential marker proteins for the assessment of irradiation-induced cytosolic RBC lesions.
CONCLUSIONS AND CLINICAL RELEVANCE: Gamma irradiation and subsequent ex vivo storage according to the Council of Europe guidelines were found to affect RBC protein structures. The validated marker proteins can serve as a basis for the development of a screening assay to monitor the quality of irradiated RBC concentrates during ex vivo storage.
Original language | English |
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Journal | Proteomics. Clinical applications |
Volume | 7 |
Issue number | 7-8 |
Pages (from-to) | 561-570 |
Number of pages | 10 |
DOIs | |
Publication status | Published - 01.08.2013 |
Research areas and keywords
- Adult
- Biological Markers
- Chromatography, Liquid
- Cytosol
- Erythrocytes
- Female
- Gamma Rays
- Humans
- Male
- Mass Spectrometry
- Nanotechnology
- Proteomics
- Specimen Handling
- Time Factors
- Two-Dimensional Difference Gel Electrophoresis
- Young Adult