ERβ-specific agonists and genistein inhibit proliferation and induce apoptosis in the large and small intestine

Burkhardt Schleipen, Torsten Hertrampf, K H Fritzemeier, Felix Kluxen, A Lorenz, Almut Molzberger, Martina Velders, Patrick Rene Diel

Publication: Contribution to journalJournal articlesResearchpeer-review

Abstract

Epidemiological data indicate that intake of estrogens and isoflavones may be beneficial for the prevention of colorectal cancer (CRC). Based on this data, the aim of the study was to investigate estrogen receptor (ER) subtype-specific effects on intestinal homeostasis. Ovariectomized (OVX) female Wistar rats were either treated with 17β-estradiol (4 μg/kg body wt/day) (E2), an ERα-specific agonist (ALPHA) (10 μg/kg body wt/day), an ERβ-specific agonist (BETA) (100 μg/kg body wt/day) or genistein (GEN) (10 mg/kg body wt/day) for three weeks. Vehicle-treated OVX and SHAM animals and those cotreated with BETA and the pure antiestrogen Fulvestrant (ICI 182780) (100 μg/kg body wt/day and 3 mg/kg body wt/day) served as controls. GEN and BETA treatment but not E2 and ALPHA administration reduced proliferation in ileal and colonic mucosa cells. The rate of apoptosis in the small intestine and colon was increased by treatment with BETA and GEN, but not by E2. BETA induced antiproliferative and proapoptotic activity also in SHAM animals. The effects were antagonized by the pure antiestrogen Fulvestrant. Polymerase chain reaction gene array analysis revealed that BETA resulted in the downregulation of the oncogene transformation-related protein 63 (p63). Our data indicate that activation of the ERβ by specific ERβ agonists and GEN induces antiproliferative and proapoptotic effects in the intestinal tract. This observation can be taken as an indication that intake of GEN and specific ERβ agonists may protect the ileal and colonic epithelium from tumor development via modulation of tissue homeostasis.

Original languageEnglish
JournalCarcinogenesis
Volume32
Issue number11
Pages (from-to)1675-1683
Number of pages9
DOIs
Publication statusPublished - 01.11.2011

Research areas and keywords

  • Animals
  • Anticarcinogenic Agents
  • Apoptosis
  • Biological Markers
  • Blotting, Western
  • Cell Proliferation
  • Drug Synergism
  • Estradiol
  • Estrogen Antagonists
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Estrogens
  • Female
  • Gene Expression Profiling
  • Genistein
  • Immunoenzyme Techniques
  • Intestine, Large
  • Intestine, Small
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction

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