Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway

Research output: Contribution to journal › Journal articles › Research › peer-review

Standard

Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology : Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway. / Nader, Elie; Grau, Marijke; Fort, Romain; Collins, Bianca; Cannas, Giovanna; Gauthier, Alexandra; Walpurgis, Katja; Martin, Cyril; Bloch, Wilhelm; Poutrel, Solène; Hot, Arnaud; Renoux, Céline; Thevis, Mario; Joly, Philippe; Romana, Marc; Guillot, Nicolas; Connes, Philippe.

In: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 81, 01.12.2018, p. 28-35.

Research output: Contribution to journal › Journal articles › Research › peer-review

Harvard

Nader, E, Grau, M, Fort, R, Collins, B, Cannas, G, Gauthier, A, Walpurgis, K, Martin, C, Bloch, W, Poutrel, S, Hot, A, Renoux, C, Thevis, M, Joly, P, Romana, M, Guillot, N & Connes, P 2018, 'Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway', Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, vol. 81, pp. 28-35. https://doi.org/10.1016/j.niox.2018.10.003

APA

Nader, E., Grau, M., Fort, R., Collins, B., Cannas, G., Gauthier, A., Walpurgis, K., Martin, C., Bloch, W., Poutrel, S., Hot, A., Renoux, C., Thevis, M., Joly, P., Romana, M., Guillot, N., & Connes, P. (2018). Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, 81, 28-35. https://doi.org/10.1016/j.niox.2018.10.003

Vancouver

Nader E, Grau M, Fort R, Collins B, Cannas G, Gauthier A et al. Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society. 2018 Dec 1;81:28-35. https://doi.org/10.1016/j.niox.2018.10.003

Bibtex

@article{ae7e4404e9fc481f930f7a39f9e99d05,
title = "Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway",
abstract = "Hydroxyurea (HU) has been suggested to act as a nitric oxide (NO) donor in sickle cell anemia (SCA). However, little is known about the HU NO-related effects on red blood cell (RBC) physiology and NO signalling pathway. Thirty-four patients with SCA (22 under HU treatment (HU+) and 12 without (HU-)) and 17 healthy subjects (AA) were included. RBC nitrite content, deformability and reactive oxygen species (ROS) levels were measured. RBC NO-synthase (RBC-NOS) signalling pathway was assessed by the measurement of RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation. We also investigated the in vitro effects of Sodium Nitroprusside (SNP), a NO donor, on the same parameters in SCA RBC. RBC nitrite content was higher in HU+ than in HU- and AA. RBC deformability was decreased in SCA patients compared to AA but the decrease was more pronounced in HU-. RBC ROS level was increased in SCA compared to AA but the level was higher in HU- than in HU+. RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation were decreased in HU+ compared to HU- and AA. SCA RBC treated with SNP showed increased deformability, reduced ROS content and a decrease in AKT and RBC-NOS phosphorylation. Our study suggests that HU, through its effects on foetal hemoglobin and possibly on NO delivery, would modulate RBC NO signalling pathway, RBC rheology and oxidative stress.",
keywords = "Journal Article",
author = "Elie Nader and Marijke Grau and Romain Fort and Bianca Collins and Giovanna Cannas and Alexandra Gauthier and Katja Walpurgis and Cyril Martin and Wilhelm Bloch and Sol{\`e}ne Poutrel and Arnaud Hot and C{\'e}line Renoux and Mario Thevis and Philippe Joly and Marc Romana and Nicolas Guillot and Philippe Connes",
note = "Copyright {\textcopyright} 2018 Elsevier Inc. All rights reserved.",
year = "2018",
month = dec,
day = "1",
doi = "10.1016/j.niox.2018.10.003",
language = "English",
volume = "81",
pages = "28--35",
journal = "Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society",
issn = "1089-8611",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology

T2 - Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway

AU - Nader, Elie

AU - Grau, Marijke

AU - Fort, Romain

AU - Collins, Bianca

AU - Cannas, Giovanna

AU - Gauthier, Alexandra

AU - Walpurgis, Katja

AU - Martin, Cyril

AU - Bloch, Wilhelm

AU - Poutrel, Solène

AU - Hot, Arnaud

AU - Renoux, Céline

AU - Thevis, Mario

AU - Joly, Philippe

AU - Romana, Marc

AU - Guillot, Nicolas

AU - Connes, Philippe

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Hydroxyurea (HU) has been suggested to act as a nitric oxide (NO) donor in sickle cell anemia (SCA). However, little is known about the HU NO-related effects on red blood cell (RBC) physiology and NO signalling pathway. Thirty-four patients with SCA (22 under HU treatment (HU+) and 12 without (HU-)) and 17 healthy subjects (AA) were included. RBC nitrite content, deformability and reactive oxygen species (ROS) levels were measured. RBC NO-synthase (RBC-NOS) signalling pathway was assessed by the measurement of RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation. We also investigated the in vitro effects of Sodium Nitroprusside (SNP), a NO donor, on the same parameters in SCA RBC. RBC nitrite content was higher in HU+ than in HU- and AA. RBC deformability was decreased in SCA patients compared to AA but the decrease was more pronounced in HU-. RBC ROS level was increased in SCA compared to AA but the level was higher in HU- than in HU+. RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation were decreased in HU+ compared to HU- and AA. SCA RBC treated with SNP showed increased deformability, reduced ROS content and a decrease in AKT and RBC-NOS phosphorylation. Our study suggests that HU, through its effects on foetal hemoglobin and possibly on NO delivery, would modulate RBC NO signalling pathway, RBC rheology and oxidative stress.

AB - Hydroxyurea (HU) has been suggested to act as a nitric oxide (NO) donor in sickle cell anemia (SCA). However, little is known about the HU NO-related effects on red blood cell (RBC) physiology and NO signalling pathway. Thirty-four patients with SCA (22 under HU treatment (HU+) and 12 without (HU-)) and 17 healthy subjects (AA) were included. RBC nitrite content, deformability and reactive oxygen species (ROS) levels were measured. RBC NO-synthase (RBC-NOS) signalling pathway was assessed by the measurement of RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation. We also investigated the in vitro effects of Sodium Nitroprusside (SNP), a NO donor, on the same parameters in SCA RBC. RBC nitrite content was higher in HU+ than in HU- and AA. RBC deformability was decreased in SCA patients compared to AA but the decrease was more pronounced in HU-. RBC ROS level was increased in SCA compared to AA but the level was higher in HU- than in HU+. RBC-NOS serine1177 and RBC-AKT serine473 phosphorylation were decreased in HU+ compared to HU- and AA. SCA RBC treated with SNP showed increased deformability, reduced ROS content and a decrease in AKT and RBC-NOS phosphorylation. Our study suggests that HU, through its effects on foetal hemoglobin and possibly on NO delivery, would modulate RBC NO signalling pathway, RBC rheology and oxidative stress.

KW - Journal Article

U2 - 10.1016/j.niox.2018.10.003

DO - 10.1016/j.niox.2018.10.003

M3 - Journal articles

C2 - 30342855

VL - 81

SP - 28

EP - 35

JO - Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society

JF - Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society

SN - 1089-8611

ER -

ID: 3554324