Investigations in carbon isotope ratios of seized testosterone and boldenone preparations

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Investigations in carbon isotope ratios of seized testosterone and boldenone preparations. / Piper, Thomas; Thevis, Mario.

In: Drug testing and analysis, 30.06.2021.

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@article{7093758b9eae46a89bb494ad30da5390,
title = "Investigations in carbon isotope ratios of seized testosterone and boldenone preparations",
abstract = "In order to detect the misuse of testosterone (T) or boldenone (Bo) in doping control analysis, the confirmation of atypical findings employing the determination of carbon isotope ratios (CIR) is mandatory for issuing adverse analytical findings. Elevated concentrations of T (or elevated T/epitestosterone ratios) may result from confounding factors such as ethanol intake, and the presence of low urinary concentrations of Bo can originate from endogenous or urinary in situ production of small amounts of the steroid. As pharmaceutical preparations of Bo and T are generally depleted in 13 C, their CIR differ significantly from the 13 C-enriched endogenous steroids. Some rare cases have been reported on pharmaceutical preparations showing 13 C-enriched isotope ratios that complicate the current application of CIR in sports drug testing. Therefore, the CIR of a subset of n = 157 T preparations and n = 39 Bo preparations seized in Switzerland and Germany between 2013 and 2018 was analyzed in order to estimate the possible impact of steroid preparations showing 13 C-enriched isotope ratios on the current approach to detect their misuse. All investigated Bo preparations showed CIR in the expected range between - 26.7 and -30.3 ‰. Within the T samples, 95 % showed the expected values below -26 ‰ while 6 samples fall between -25 and -26 ‰ and one sample was indistinguishable from endogenously produced T with a CIR of -23.3 ‰.",
author = "Thomas Piper and Mario Thevis",
note = "This article is protected by copyright. All rights reserved.",
year = "2021",
month = jun,
day = "30",
doi = "10.1002/dta.3120",
language = "English",
journal = "Drug testing and analysis",
issn = "1942-7603",
publisher = "John Wiley & Sons, Ltd",

}

RIS

TY - JOUR

T1 - Investigations in carbon isotope ratios of seized testosterone and boldenone preparations

AU - Piper, Thomas

AU - Thevis, Mario

N1 - This article is protected by copyright. All rights reserved.

PY - 2021/6/30

Y1 - 2021/6/30

N2 - In order to detect the misuse of testosterone (T) or boldenone (Bo) in doping control analysis, the confirmation of atypical findings employing the determination of carbon isotope ratios (CIR) is mandatory for issuing adverse analytical findings. Elevated concentrations of T (or elevated T/epitestosterone ratios) may result from confounding factors such as ethanol intake, and the presence of low urinary concentrations of Bo can originate from endogenous or urinary in situ production of small amounts of the steroid. As pharmaceutical preparations of Bo and T are generally depleted in 13 C, their CIR differ significantly from the 13 C-enriched endogenous steroids. Some rare cases have been reported on pharmaceutical preparations showing 13 C-enriched isotope ratios that complicate the current application of CIR in sports drug testing. Therefore, the CIR of a subset of n = 157 T preparations and n = 39 Bo preparations seized in Switzerland and Germany between 2013 and 2018 was analyzed in order to estimate the possible impact of steroid preparations showing 13 C-enriched isotope ratios on the current approach to detect their misuse. All investigated Bo preparations showed CIR in the expected range between - 26.7 and -30.3 ‰. Within the T samples, 95 % showed the expected values below -26 ‰ while 6 samples fall between -25 and -26 ‰ and one sample was indistinguishable from endogenously produced T with a CIR of -23.3 ‰.

AB - In order to detect the misuse of testosterone (T) or boldenone (Bo) in doping control analysis, the confirmation of atypical findings employing the determination of carbon isotope ratios (CIR) is mandatory for issuing adverse analytical findings. Elevated concentrations of T (or elevated T/epitestosterone ratios) may result from confounding factors such as ethanol intake, and the presence of low urinary concentrations of Bo can originate from endogenous or urinary in situ production of small amounts of the steroid. As pharmaceutical preparations of Bo and T are generally depleted in 13 C, their CIR differ significantly from the 13 C-enriched endogenous steroids. Some rare cases have been reported on pharmaceutical preparations showing 13 C-enriched isotope ratios that complicate the current application of CIR in sports drug testing. Therefore, the CIR of a subset of n = 157 T preparations and n = 39 Bo preparations seized in Switzerland and Germany between 2013 and 2018 was analyzed in order to estimate the possible impact of steroid preparations showing 13 C-enriched isotope ratios on the current approach to detect their misuse. All investigated Bo preparations showed CIR in the expected range between - 26.7 and -30.3 ‰. Within the T samples, 95 % showed the expected values below -26 ‰ while 6 samples fall between -25 and -26 ‰ and one sample was indistinguishable from endogenously produced T with a CIR of -23.3 ‰.

U2 - 10.1002/dta.3120

DO - 10.1002/dta.3120

M3 - Journal articles

C2 - 34192821

JO - Drug testing and analysis

JF - Drug testing and analysis

SN - 1942-7603

ER -

ID: 6071054