Mass spectrometry-based analysis of IGF-1 and hGH

Mario Thevis, Michael Bredehöft, Maxie Kohler, Wilhelm Schänzer

Publication: Contribution to journalJournal articlesResearch

Abstract

Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.

Original languageEnglish
JournalHandbook of experimental pharmacology
Issue number195
Pages (from-to)201-207
Number of pages7
ISSN0171-2004
DOIs
Publication statusPublished - 01.01.2010

Research areas and keywords

  • Amino Acid Sequence
  • Doping in Sports
  • Electrophoresis, Gel, Two-Dimensional
  • Human Growth Hormone
  • Humans
  • Insulin-Like Growth Factor I
  • Mass Spectrometry
  • Molecular Sequence Data
  • Recombinant Proteins

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