Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting

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  • Miriam Capri
  • Cristina Morsiani
  • Aurelia Santoro
  • Manuela Moriggi
  • Maria Conte
  • Morena Martucci
  • Elena Bellavista
  • Cristina Fabbri
  • Enrico Giampieri
  • Martin Flück
  • Severin Ruoss
  • Lorenza Brocca
  • Monica Canepari
  • Emanuela Longa
  • Irene Di Giulio
  • Roberto Bottinelli
  • Paolo Cerretelli
  • Stefano Salvioli
  • Cecilia Gelfi
  • Claudio Franceschi
  • Marco Narici
  • Jörn Rittweger

Research units


The Sarcolab pilot study of 2 crewmembers, investigated before and after a 6-mo International Space Station mission, has demonstrated the substantial muscle wasting and weakness, along with disruption of muscle's oxidative metabolism. The present work aimed at evaluating the pro/anti-inflammatory status in the same 2 crewmembers (A, B). Blood circulating (c-)microRNAs (miRs), c-proteasome, c-mitochondrial DNA, and cytokines were assessed by real-time quantitative PCR or ELISA tests. Time series analysis was performed ( i.e., before flight and after landing) at 1 and 15 d of recovery (R+1 and R+15, respectively). C-biomarkers were compared with an age-matched control population and with two-dimensional proteomic analysis of the 2 crewmembers' muscle biopsies. Striking differences were observed between the 2 crewmembers at R+1, in terms of inflamma-miRs (c-miRs-21-5p, -126-3p, and -146a-5p), muscle specific (myo)-miR-206, c-proteasome, and IL-6/leptin, thus making the 2 astronauts dissimilar to each other. Final recovery levels of c-proteasome, c-inflamma-miRs, and c-myo-miR-206 were not reverted to the baseline values in crewmember A. In both crewmembers, myo-miR-206 changed significantly after recovery. Muscle biopsy of astronaut A showed an impressive 80% increase of α-1-antitrypsin, a target of miR-126-3p. These results point to a strong stress response induced by spaceflight involving muscle tissue and the proinflammatory setting, where inflamma-miRs and myo-miR-206 mediate the systemic recovery phase after landing.-Capri, M., Morsiani, C., Santoro, A., Moriggi, M., Conte, M., Martucci, M., Bellavista, E., Fabbri, C., Giampieri, E., Albracht, K., Flück, M., Ruoss, S., Brocca, L., Canepari, M., Longa, E., Di Giulio, I., Bottinelli, R., Cerretelli, P., Salvioli, S., Gelfi, C., Franceschi, C., Narici, M., Rittweger, J. Recovery from six-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting.

Original languageEnglish
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Issue number4
Pages (from-to)5168-5180
Number of pages13
Publication statusPublished - 04.2019

ID: 3565490


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