Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis

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Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis. / Göschl, Lorenz; Gmeiner, Günter; Gärtner, Peter et al.

In: Drug testing and analysis, Vol. 13, No. 9, 09.09.2021, p. 1668-1677.

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@article{7a9a5434ef04497d9937e403bbb04be9,
title = "Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis",
abstract = "The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.",
keywords = "anabolic androgenic steroids, glucuronide, high-resolution mass spectrometry, phase-II metabolite, stanozolol",
author = "Lorenz G{\"o}schl and G{\"u}nter Gmeiner and Peter G{\"a}rtner and Georg Stadler and Valentin Enev and Mario Thevis and Wilhelm Sch{\"a}nzer and Sven Guddat and Guro Forsdahl",
note = "{\textcopyright} 2021 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.",
year = "2021",
month = sep,
day = "9",
doi = "10.1002/dta.3109",
language = "English",
volume = "13",
pages = "1668--1677",
journal = "Drug testing and analysis",
issn = "1942-7603",
publisher = "John Wiley & Sons, Ltd",
number = "9",

}

RIS

TY - JOUR

T1 - Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis

AU - Göschl, Lorenz

AU - Gmeiner, Günter

AU - Gärtner, Peter

AU - Stadler, Georg

AU - Enev, Valentin

AU - Thevis, Mario

AU - Schänzer, Wilhelm

AU - Guddat, Sven

AU - Forsdahl, Guro

N1 - © 2021 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.

PY - 2021/9/9

Y1 - 2021/9/9

N2 - The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

AB - The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

KW - anabolic androgenic steroids

KW - glucuronide

KW - high-resolution mass spectrometry

KW - phase-II metabolite

KW - stanozolol

UR - https://www.mendeley.com/catalogue/a5dfa7bb-b9ad-3eae-98c5-0cccc3aa5fc9/

U2 - 10.1002/dta.3109

DO - 10.1002/dta.3109

M3 - Journal articles

C2 - 34089570

VL - 13

SP - 1668

EP - 1677

JO - Drug testing and analysis

JF - Drug testing and analysis

SN - 1942-7603

IS - 9

ER -

ID: 6059742