Temporal indication of cannabis use by means of THC glucuronide determination

Ute Mareck, Nadine Haenelt, Hans Geyer, Sven Guddat, Matthias Kamber, Rudolf Brenneisen, Mario Thevis, Wilhelm Schänzer

Publication: Contribution to journalJournal articlesResearch

Abstract

According to the regulations of the World Anti-Doping Agency (WADA), the use of cannabinoids is forbidden in competition. In doping controls, the detection of cannabinoid misuse is based on the analysis of the non-psychoactive metabolite 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (carboxy-THC). The determination of values greater than 15 ng/mL in urine represents an adverse analytical finding; however, no accurate prediction of the time of application is possible as the half-life of carboxy-THC ranges between three and four days. Consequently the detection of carboxy-THC in doping control urine samples collected in competition might also result from cannabis use in out-of-competition periods. The analysis of the glucuronide of the pharmacologically active delta 9-tetrahydrocannabinol (THC-gluc) may represent a complementary indicator for the detection of cannabis misuse in competition.An assay for the determination of THC-gluc in human urine was established. The sample preparation consisted of liquid-liquid extraction of urine specimens, and extracts were analysed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Authentic doping-control urine samples as well as specimens obtained from a controlled smoking study were analysed and assay characteristics such as specificity, detection limit (0.1 ng/mL), precision (>90%), recovery ( approximately 80%), and extraction efficiency (90%) were determined.

Original languageEnglish
JournalDrug testing and analysis
Volume1
Issue number11-12
Pages (from-to)505-510
Number of pages6
DOIs
Publication statusPublished - 01.11.2009

Research areas and keywords

  • Chromatography, Liquid
  • Doping in Sports
  • Dronabinol
  • Glucuronides
  • Humans
  • Marijuana Smoking
  • Sensitivity and Specificity
  • Substance Abuse Detection
  • Tandem Mass Spectrometry
  • Time Factors

Fingerprint

Dive into the research topics of 'Temporal indication of cannabis use by means of THC glucuronide determination'. Together they form a unique fingerprint.

Citation