The fungus Cunninghamella elegans can produce human and equine metabolites of selective androgen receptor modulators (SARMs)

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The fungus Cunninghamella elegans can produce human and equine metabolites of selective androgen receptor modulators (SARMs). / Rydevik, Axel; Thevis, Mario; Krug, Oliver; Bondesson, Ulf; Hedeland, Mikael.

In: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 43, No. 5, 01.05.2013, p. 409-420.

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@article{45fd88497b7a4c05aa09936e131ef3bd,
title = "The fungus Cunninghamella elegans can produce human and equine metabolites of selective androgen receptor modulators (SARMs)",
abstract = "1. Selective androgen receptor modulators (SARMs) are a group of substances that have potential to be used as doping agents in sports. Being a relatively new group not available on the open market means that no reference materials are commercially available for the main metabolites. In the presented study, the in vitro metabolism of SARMs by the fungus Cunninghamella elegans has been investigated with the purpose of finding out if it can produce relevant human and equine metabolites. 2. Three different SARMs, S1, S4 and S24, were incubated for 5 days with C. elegans. The samples were analysed both with and without sample pretreatment using ultra performance liquid chromatography coupled to high resolution mass spectrometry. 3. All the important phase I and some phase II metabolites from human and horse were formed by the fungus. They were formed through reactions such as hydroxylation, deacetylation, O-dephenylation, nitro-reduction, acetylation and sulfonation. 4. The study showed that the fungus produced relevant metabolites of the SARMs and thus can be used to mimic mammalian metabolism. Furthermore, it has the potential to be used for future production of reference material.",
keywords = "Androgens, Animals, Cunninghamella, Doping in Sports, Horses, Humans, Performance-Enhancing Substances, Receptors, Androgen, Substance Abuse Detection",
author = "Axel Rydevik and Mario Thevis and Oliver Krug and Ulf Bondesson and Mikael Hedeland",
year = "2013",
month = "5",
day = "1",
doi = "10.3109/00498254.2012.729102",
language = "English",
volume = "43",
pages = "409--420",
journal = "Xenobiotica; the fate of foreign compounds in biological systems",
issn = "1366-5928",
publisher = "Informa Healthcare",
number = "5",

}

RIS

TY - JOUR

T1 - The fungus Cunninghamella elegans can produce human and equine metabolites of selective androgen receptor modulators (SARMs)

AU - Rydevik, Axel

AU - Thevis, Mario

AU - Krug, Oliver

AU - Bondesson, Ulf

AU - Hedeland, Mikael

PY - 2013/5/1

Y1 - 2013/5/1

N2 - 1. Selective androgen receptor modulators (SARMs) are a group of substances that have potential to be used as doping agents in sports. Being a relatively new group not available on the open market means that no reference materials are commercially available for the main metabolites. In the presented study, the in vitro metabolism of SARMs by the fungus Cunninghamella elegans has been investigated with the purpose of finding out if it can produce relevant human and equine metabolites. 2. Three different SARMs, S1, S4 and S24, were incubated for 5 days with C. elegans. The samples were analysed both with and without sample pretreatment using ultra performance liquid chromatography coupled to high resolution mass spectrometry. 3. All the important phase I and some phase II metabolites from human and horse were formed by the fungus. They were formed through reactions such as hydroxylation, deacetylation, O-dephenylation, nitro-reduction, acetylation and sulfonation. 4. The study showed that the fungus produced relevant metabolites of the SARMs and thus can be used to mimic mammalian metabolism. Furthermore, it has the potential to be used for future production of reference material.

AB - 1. Selective androgen receptor modulators (SARMs) are a group of substances that have potential to be used as doping agents in sports. Being a relatively new group not available on the open market means that no reference materials are commercially available for the main metabolites. In the presented study, the in vitro metabolism of SARMs by the fungus Cunninghamella elegans has been investigated with the purpose of finding out if it can produce relevant human and equine metabolites. 2. Three different SARMs, S1, S4 and S24, were incubated for 5 days with C. elegans. The samples were analysed both with and without sample pretreatment using ultra performance liquid chromatography coupled to high resolution mass spectrometry. 3. All the important phase I and some phase II metabolites from human and horse were formed by the fungus. They were formed through reactions such as hydroxylation, deacetylation, O-dephenylation, nitro-reduction, acetylation and sulfonation. 4. The study showed that the fungus produced relevant metabolites of the SARMs and thus can be used to mimic mammalian metabolism. Furthermore, it has the potential to be used for future production of reference material.

KW - Androgens

KW - Animals

KW - Cunninghamella

KW - Doping in Sports

KW - Horses

KW - Humans

KW - Performance-Enhancing Substances

KW - Receptors, Androgen

KW - Substance Abuse Detection

U2 - 10.3109/00498254.2012.729102

DO - 10.3109/00498254.2012.729102

M3 - Journal articles

C2 - 23153056

VL - 43

SP - 409

EP - 420

JO - Xenobiotica; the fate of foreign compounds in biological systems

JF - Xenobiotica; the fate of foreign compounds in biological systems

SN - 1366-5928

SN - 0049-8254

IS - 5

ER -

ID: 129243